Cellular Metabolic Dynamics

New Interest Group!

Join us for engaging discussions and learning on Cellular Metabolic Dynamics.


All are welcome!

We hope to encourage those researchers interested in cellular metabolism to participate in an open informal meetings regarding:

 

1) cell metabolism

2) metabolism in cancer

3) metabolism in development

4) metabolism in neurodegenerative diseases

5) fluorescence lifetime imaging  microcopy (FLIM)

6) Seahorse technologies

7) new computational tools to quantify concentrations of NADH

8) new computational tools to obtain indexes of metabolic chances for drug screening, healthy and unhealthy cells

9) advances in auto-fluorescence dynamics (retenoic acid, NADH etc)

Time and Day: 8:30-9:30AM; dates below

Location: Room 3201 Natural Sciences II

Tentative schedule:

Speaker Date Topic Location
Aimee Edinger

April 14th     

8:30-9:30AM

Warburg effect and how cancer cells acquire nutrients and respond to nutrient stress and present from FLIM data 3201 Natural Sciences II
Jenu Chacko April 21st 8:30-9:30AM FLIM in tissues 3201 Natural Sciences II
Nik Hedde and Rachel Cinco

May 5th

8:30-9:30AM

Application of FLIM to defining Ovarian Follicular Metabolism; NADH Dynamics 3201 Natural Sciences II
Olga V. Razorena

June 16th 8:30-9:30AM CANCELLED

The role of CDCP1 in lipid metabolism in breast cancer cells  3201 Natural Sciences II

 

If you would like to volunteer to lead a discussion, please send Dr. Michelle Digman an email: mdigman@uci.edu

Why is this group formed?

1)  It has come to our attention that many investigators on campus are doing work in investigating changes of cellular metabolism.

  • One method used to measure the redox state of cells is the popular Seahorse technology that uses test kits to measure energy utilization in living cells. In particular this system measures “oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 5-8 minutes. OCR is an indicator of mitochondrial respiration, and ECAR is largely the result of glycolysis” (http://www.seahorsebio.com/products/how-xf-works.php ) .
  • While the Seahorse system provide bulk rate measurements some investigators are doing parallel experiments using fluorescence lifetime imaging microcopy (FLIM). The advantage of this method is that researchers have a map of metabolic changes within a cell. Thus single cell analysis can be done more accurately and comparative results can be done to distinguish cell populations at the single cell level without using any fluorescence markers.  That’s right the cells have their fluorescence biomarkers (in particular NADH, a metabolic co-factor) that is predominant in cells. Given that we are mainly exciting NADH with the 2-photon laser with a narrow emission filter between 420-465nm,, their phasor finger printing would be outside the linear phasor trajectory for free and bound NADH.
  • We will discuss topics in metabolism including Cancer, embryo development, advances in FLIM analysis and other research that focus on metabolic changes. We will also discuss other optical imaging technologies that can be used in parallel for FLIM. These include but are not limited to: Second harmonic imaging, third harmonic imaging and CARS.

 

 

2011 Cellular Metabolic Dynamics. Copyright © 2013. The Center for Complex Biological Systems, UC Irvine
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